Receptor-binding domain of SARS-Cov spike protein: soluble expression in E. coli, purification and functional characterization.
Identifieur interne : 004685 ( Main/Exploration ); précédent : 004684; suivant : 004686Receptor-binding domain of SARS-Cov spike protein: soluble expression in E. coli, purification and functional characterization.
Auteurs : Jing Chen [République populaire de Chine] ; Lin Miao ; Jia-Ming Li ; Yan-Ying Li ; Qing-Yu Zhu ; Chang-Lin Zhou ; Hong-Qing Fang ; Hui-Peng ChenSource :
- World journal of gastroenterology [ 1007-9327 ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Escherichia coli, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Plasmides, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Régulation de l'expression des gènes viraux, Solubilité, Structure tertiaire des protéines, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (virologie), Vaccins antiviraux (), Vaccins antiviraux (génétique), Virus du SRAS (génétique).
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Vaccins antiviraux, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Cellules Vero, Escherichia coli, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Plasmides, Protéines de l'enveloppe virale, Régulation de l'expression des gènes viraux, Solubilité, Structure tertiaire des protéines, Syndrome respiratoire aigu sévère, Vaccins antiviraux.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Escherichia coli, Gene Expression Regulation, Viral, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Plasmids, Protein Structure, Tertiary, SARS Virus (genetics), Severe Acute Respiratory Syndrome (prevention & control), Severe Acute Respiratory Syndrome (virology), Solubility, Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Vaccines (chemistry), Viral Vaccines (genetics).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins, Viral Vaccines.
- genetics : SARS Virus.
- prevention & control : Severe Acute Respiratory Syndrome.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Escherichia coli, Gene Expression Regulation, Viral, Plasmids, Protein Structure, Tertiary, Solubility, Spike Glycoprotein, Coronavirus, Vero Cells.
Abstract
Spike protein of coronavirus is responsible for virus binding, fusion and entry, and is a major inducer of neutralizing antibodies. This paper was to find a soluble and functional recombinant receptor-binding domain of severe acute respiratory syndrome-associated coronavirus (SARS-Cov), and to analyze its receptor binding ability.
DOI: 10.3748/wjg.v11.i39.6159
PubMed: 16273643
Affiliations:
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Le document en format XML
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sort="Zhu, Qing Yu" uniqKey="Zhu Q" first="Qing-Yu" last="Zhu">Qing-Yu Zhu</name></author><author><name sortKey="Zhou, Chang Lin" sort="Zhou, Chang Lin" uniqKey="Zhou C" first="Chang-Lin" last="Zhou">Chang-Lin Zhou</name></author><author><name sortKey="Fang, Hong Qing" sort="Fang, Hong Qing" uniqKey="Fang H" first="Hong-Qing" last="Fang">Hong-Qing Fang</name></author><author><name sortKey="Chen, Hui Peng" sort="Chen, Hui Peng" uniqKey="Chen H" first="Hui-Peng" last="Chen">Hui-Peng Chen</name></author></analytic><series><title level="j">World journal of gastroenterology</title><idno type="ISSN">1007-9327</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Escherichia coli</term><term>Gene Expression Regulation, Viral</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Plasmids</term><term>Protein Structure, Tertiary</term><term>SARS Virus (genetics)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Solubility</term><term>Spike Glycoprotein, Coronavirus</term><term>Vero Cells</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Vaccines (chemistry)</term><term>Viral Vaccines (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Escherichia coli</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (génétique)</term><term>Plasmides</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Régulation de l'expression des gènes viraux</term><term>Solubilité</term><term>Structure 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